Anti-inflammatory Activities of an Ethanol Extract of Ecklonia stolonifera in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophage Cells

详细信息    查看全文

• 作者：Min-Sup Lee ; Mi-Sung Kwon ; Ji-Woong Choi ; Taisun Shin ; Hong Kyoon No ; Jae-Sue Choi ; Dae-Seok Byun ; Jae-Il Kim ; Hyeung-Rak Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：September 12, 2012
• 年：2012
• 卷：60
• 期：36
• 页码：9120-9129
• 全文大小：420K
• 年卷期：v.60,no.36(September 12, 2012)
• ISSN：1520-5118

文摘

Ecklonia stolonifera is a brown alga that was shown to have antioxidant, anti-inflammatory, tyrosinase inhibitory, and chemopreventive activities. However, the molecular mechanisms underlying its anti-inflammatory activity remain unclear. In this study, we investigated the molecular mechanism of the anti-inflammatory action of E. stolonifera ethanolic extracts (ESE) using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. ESE inhibited LPS-induced nitric oxide (IC₅₀ = 72 卤 1.9 渭g/mL) and prostaglandin E₂ (IC₅₀ = 98 卤 5.3 渭g/mL) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 cells. ESE also reduced the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. LPS-induced nuclear factor-魏B (NF-魏B) transcriptional activity and NF-魏B translocation into the nucleus were significantly inhibited by ESE treatment through the prevention of the degradation of inhibitor 魏B-伪. Moreover, ESE inhibited the activation of Akt, ERK, JNK1/2, and p38 MAPK in LPS-stimulated RAW 264.7 cells. The main components with anti-inflammatory activity in ESE were identified as phlorofucofuroeckol A and B based on the inhibition of NO production. Our results indicate that ESE can be considered as a potential source of therapeutic agents for inflammatory diseases.

Keywords:

Ecklonia stolonifera; anti-inflammation; NF-魏B; MAP kinases; RAW 264.7 cells