Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins

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• 作者：Wensong Li ; Liangrong Yang ; Huacong Zhou ; Xiaopei Li ; Fuchun Wang ; Xingfu Yang ; Huizhou Liu
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：November 20, 2013
• 年：2013
• 卷：52
• 期：46
• 页码：16314-16320
• 全文大小：461K
• 年卷期：v.52,no.46(November 20, 2013)
• ISSN：1520-5045

文摘

In this study, gas-assisted superparamagnetic extraction (GASE) with potential large-scale application was proposed for selective separation of multicomponent proteins. Magnetic poly(glycidyl methacrylate)鈥搃minodiacetic acid鈥揨n²⁺ microspheres (MPMs) for selective separation of bovine serum albumin (BSA) and bovine hemoglobin (BHb) are selected as a model. The feasibility of flotation for concentrating BHb-loaded MPMs from dilute mixed proteins solution was proved. The well-concentrated solution of BHb-loaded MPMs could be achieved by simple adjustment of pH values and ionic strength in low regions, without additive detergent. Impurity BSA had the effects of improving the flotability of BHb-loaded MPMs, acting as foaming agent, but decreasing the enrichment ratio in the GASE process. The flotation conditions and the selective adsorption ones showed strong consistency. Under the optimal conditions, the enrichment ratio of 39 and recovery percentage of 98% was obtained within 1.3 min. Furthermore, the enrichment ratio could be further improved by added flotation steps.