Graphene Quantum Dot–MnO2 Nanosheet Based Optical Sensing Platform: A Sensitive Fluorescence “Turn Off–On” Nanosensor for Glutathione Detection and Intracellular Imaging

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• 作者：Xu Yan ; Yang Song ; Chengzhou Zhu ; Junhua Song ; Dan Du ; Xingguang Su ; Yuehe Lin
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：August 31, 2016
• 年：2016
• 卷：8
• 期：34
• 页码：21990-21996
• 全文大小：490K
• 年卷期：0
• ISSN：1944-8252

文摘

Glutathione (GSH) monitoring has attracted extensive attention because it serves a vital role in human pathologies. Herein, a convenient fluorescence “turn off–on” nanosensor based on graphene quantum dots (GQDs)–manganese dioxide (MnO₂) nanosheet has been designed for selective detection of GSH in living cells. The fluorescence intensity of GQDs can be quenched by MnO₂ nanosheets via a fluorescence resonance energy transfer. However, GSH can reduce MnO₂ nanosheets to Mn²⁺ cations and release GQDs, causing sufficient recovery of fluorescent signal. The MnO₂ nanosheets serve as both fluorescence nanoquencher and GSH recognizer in the sensing platform. The sensing platform displayed a sensitive response to GSH in the range of 0.5–10 μmol L^–1, with a detection limit of 150 nmol L⁻¹. Furthermore, the chemical response of the GQDs–MnO₂ nanoprobe exhibits high selectivity toward GSH over other electrolytes and biomolecules. Most importantly, the promising platform was successfully applied in monitoring the intracellular GSH in living cells, indicating its great potential to be used in disease diagnosis. Meanwhile, this GQDs–MnO₂ platform is also generalizable and can be easily expanded to the detection and imaging of other reactive species in living cells.