Photoswitchable Spiropyran Dyads for Biological Imaging

详细信息    查看全文

• 作者：Yaoyao Xiong ; Pablo Rivera-Fuentes ; Erdinc Sezgin ; Andreas Vargas Jentzsch ; Christian Eggeling ; Harry L. Anderson
• 刊名：Organic Letters
• 出版年：2016
• 出版时间：August 5, 2016
• 年：2016
• 卷：18
• 期：15
• 页码：3666-3669
• 全文大小：374K
• 年卷期：0
• ISSN：1523-7052

文摘

The synthesis of a small-molecule dyad consisting of a far-red-emitting silicon rhodamine dye that is covalently linked to a photochromic spironaphthothiopyran unit, which serves as a photoswitchable quencher, is reported. This system can be switched reversibly between the fluorescent and nonfluorescent states using visible light at wavelengths of 405 and 630 nm, respectively, and it works effectively in aqueous solution. Live-cell imaging demonstrates that this dyad has several desirable features, including excellent membrane permeability, fast and reversible modulation of fluorescence by visible light, and good contrast between the bright and dark states.