Visible-Light-Stimulated Enzymelike Activity of Graphene Oxide and Its Application for Facile Glucose Sensing
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- 作者：Guang-Li Wang ; Xiufang Xu ; Xiuming Wu ; Genxia Cao ; Yuming Dong ; Zaijun Li
- 刊名：Journal of Physical Chemistry C
- 出版年：2014
- 出版时间：December 4, 2014
- 年：2014
- 卷：118
- 期：48
- 页码：28109-28117
- 全文大小：403K
- ISSN：1932-7455
文摘
A novel and interesting enzyme-mimicking activity of chitosan-functionalized graphene oxide (CS-GO) upon phototriggering was demonstrated. CS-GO could catalyze oxidation of a typical chromogenic substrate (3,3鈥?5,5鈥?tetramethylbenzidine, TMB) under visible light (位 鈮?400 nm) stimulation, which was independent of destructive H2O2. Visible light triggering was a rapid, clean, and versatile means for inducing the enzymelike activity of CS-GO, which was superior to the current peroxidase or peroxidase mimetics that use H2O2 as an oxidant. Compared to the natural enzyme horseradish peroxidase (HRP), CS-GO had a higher affinity for TMB. The mechanism of the phototriggered enzyme-mimicking activity of CS-GO was investigated in detail. In addition, a novel and facile colorimetric method was realized for detection of glucose based on the photoactivated CS-GO enzyme-mimicking system and the competitive interaction of concanavalin A with chitosan or glucose. The methodology showed a linear response for glucose in the range 2.5鈥?.0 mmol/L with a detection limit as low as 0.5 渭mol/L. The sensing system was applied for detection of glucose in human serum with satisfactory results.