Metabolite Profile of Salidroside in Rats by Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry and High-Performance Liquid Chromatography Coupled with Qua
详细信息 查看全文
- 作者：Zhiwei Hu ; Ziming Wang ; Yong Liu ; Yan Wu ; Xuejiao Han ; Jian Zheng ; Xiufeng Yan ; Yang Wang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：October 21, 2015
- 年：2015
- 卷：63
- 期：41
- 页码：8999-9005
- 全文大小：399K
- ISSN：1520-5118
文摘
In the present work, the salidroside metabolite profile in rat urine was investigated, and subsequently the metabolic pathways of salidroside were proposed. After administrations of salidroside at an oral dose of 100 or 500 mg/kg, rat urine samples were collected and pretreated with methanol to precipitate the proteins. The pretreated samples were analyzed by an Acquity ultraperformance liquid chromatography (UPLC) coupled with an HSS T3 column and detected by quadrupole time-of-flight mass spectrometry (Q-TOF-MS) or high-performance liquid chromatography coupled with hybrid triple-quadrupole linear ion trap mass spectrometry (HPLC/Q-trap-MS). A total of eight metabolites were detected and identified on the basis of the characteristics of their protonated ions in the urine samples. The results elucidated that salidroside was metabolized via glucuronidation, sulfation, deglycosylation, hydroxylation, methylation, and dehydroxylation pathways in vivo.