Lab in a Tube: Sensitive Detection of MicroRNAs in Urine Samples from Bladder Cancer Patients Using a Single-Label DNA Probe with AIEgens

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• 作者：Xuehong Min ; Yuan Zhuang ; Zhenyu Zhang ; Yongmei Jia ; Abdul Hakeem ; Fuxin Zheng ; Yong Cheng ; Ben Zhong Tang ; Xiaoding Lou ; Fan Xia
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：August 5, 2015
• 年：2015
• 卷：7
• 期：30
• 页码：16813-16818
• 全文大小：329K
• ISSN：1944-8252

文摘

We demonstrate an ultrasensitive microRNA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. Furthermore, the assay could accomplish various detection limits by tuning the reaction temperature due to the different activity of exonuclease III corresponding to the diverse temperature. Specifically, 1 pM miR-21 can be detected in 40 min at 37 掳C, and 10 aM (about 300 molecules in 50 渭L) miR-21 could be discriminated in 7 days at 4 掳C. The great specificity of the assay guarantees that the real 21 urine samples from the bladder cancer patients are successfully detected by our method.