Material Properties and Cytocompatibility of Injectable MMP Degradable Poly(lactide ethylene oxide fumarate) Hydrogel as a Carrier for Marrow Stromal Cells
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- 作者：Xuezhong He ; Esmaiel Jabbari
- 刊名：Biomacromolecules
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：8
- 期：3
- 页码：780 - 792
- 全文大小：556K
- 年卷期：v.8,no.3(March 2007)
- ISSN：1526-4602
文摘
Injectable in situ crosslinkable biomaterials seeded with multipotent progenitor cells and coupled with minimallyinvasive arthroscopic techniques are an attractive alternative for treating irregularly shaped osteochondral defects.An in situ crosslinkable poly(lactide-co-ethylene oxide-co-fumarate) (PLEOF) macromer has been developedwith ultralow molecular weight poly(L-lactide) and poly(ethylene glycol) (PEG) units linked by fumaryl unit.The PLEOF macromer was crosslinked with the MMP-13 degradable peptide sequence QPQGLAK with acrylateend-groups or the methylene bisacrylamide (BISAM) crosslinker to form enzymatically or hydrolytically degradablehydrogels, respectively. Cell viability of the peptide crosslinker was significantly higher than that of BISAM.The relatively higher molecular weight peptide crosslinker significantly affected the water content and the rate ofcrosslinking (e.g., sol vs gel fraction). The addition of a small fraction of a highly reactive BISAM crosslinkerto the PLEOF/peptide mixture reduced the gelation time and increased the elastic modulus while retaining enzymaticdegradability of the hydrogel. Bone marrow stromal (BMS) cells were encapsulated in the peptide crosslinkedPLEOF hydrogel; 84% of the encapsulated cells was viable after 1 week of incubation in osteogenic media. Theencapsulated BMS cells differentiated to osteoblasts and produced a mineralized matrix, as measured by ALPaseactivity and calcium content. The degradation rate of the hydrogel depended on the ratio of the peptide to theBISAM crosslinker, MMP-13 concentration, and incubation time. The results demonstrate that the peptidecrosslinked PLEOF hydrogel with tunable degradation characteristics is potentially useful as an injectable in situcrosslinkable carrier for bone marrow stromal cells.