Photoluminescent Hyperbranched Poly(amido amine) Containing 尾-Cyclodextrin as a Nonviral Gene Delivery Vector

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• 作者：Yan Chen ; Linzhu Zhou ; Yan Pang ; Wei Huang ; Feng Qiu ; Xulin Jiang ; Xinyuan Zhu ; Deyue Yan ; Qun Chen
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：June 15, 2011
• 年：2011
• 卷：22
• 期：6
• 页码：1162-1170
• 全文大小：1037K
• 年卷期：v.22,no.6(June 15, 2011)
• ISSN：1520-4812

文摘

Hyperbranched poly(amido amine)s (HPAAs) containing different amounts of 尾-cyclodextrin (尾-CD) (HPAA-CDs) were synthesized in one-pot by Michael addition copolymerization of N,N鈥?/i>-methylene bisacrylamide, 1-(2-aminoethyl)piperazine, and mono-6-deoxy-6-ethylenediamino-尾-CD. In comparison to pure HPAA, the fluorescence intensity of HPAA-CDs was enhanced significantly while the cytotoxicity became lower. Ascribed to plenty of amino groups and strong photoluminescence, HPAA-CDs could be used as nonviral gene delivery vectors, and the corresponding gene transfection was evaluated. The experimental results indicated that HPAA-CDs condensed the plasmid DNA very well. By utilizing the fluorescent properties of HPAA-CDs, the cellular uptake and gene transfection processes were tracked by flow cytometry and confocal laser scanning microscopy without any fluorescent labeling. The transfection efficiencies of HPAA-CDs were similar to that of pure HPAA. In addition, the inner cavities of 尾-CDs in HPAA-CDs could be used to encapsulate drugs through host鈥揼uest interaction. Therefore, the HPAA-CDs may have potential application in the combination of gene therapy and chemotherapy.