Polysaccharide-Hair Cationic Polypeptide Nanogels: Self-Assembly and Enzymatic Polymerization of Amylose Primer-Modified Cholesteryl Poly(l-lysine)

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• 作者：Nobuyuki Morimoto ; Mio Yamazaki ; Junko Tamada ; Kazunari Akiyoshi
• 刊名：Langmuir
• 出版年：2013
• 出版时间：June 18, 2013
• 年：2013
• 卷：29
• 期：24
• 页码：7509-7514
• 全文大小：304K
• 年卷期：v.29,no.24(June 18, 2013)
• ISSN：1520-5827

文摘

In this study, we prepared a new associating polymer, ChMaPLL, by the substitution of the poly(l-lysine) moiety with oligosaccharide amylose primer and cholesterol. ChMaPLL formed positively charged polypeptide nanogels (50 nm) via self-assembly in water. The nanogels showed a secondary structural transition to an 伪-helix structure induced by poly(l-lysine) in response to an increase in pH. Oligosaccharides of the nanogels reacted with the phosphorylase a enzyme. Amylose-conjugated nanogels were obtained by enzymatic polymerization. The elongation of the saccharide chain shielded the positive charge of the nanogels. The multiresponsive polysaccharide鈥損olypeptide hybrid nanogels might prove to be useful in the areas of biotechnology and biomedicine.