Perfluorocarbon-Encapsulated PLGA-PEG Emulsions as Enhancement Agents for Highly Efficient Reoxygenation to Cell and Organism
详细信息 查看全文
- 作者：Yanjie Yao ; Minmin Zhang ; Tian Liu ; Juan Zhou ; Yuan Gao ; Zhengfeng Wen ; Jun Guan ; Jun Zhu ; Zhaofen Lin ; Dannong He
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2015
- 出版时间：August 26, 2015
- 年：2015
- 卷：7
- 期：33
- 页码：18369-18378
- 全文大小：545K
- ISSN：1944-8252
文摘
Perfluorocarbon (PFC), a kind of oxygen carrier, is encapsulated in PLGA-PEG to prepare a PLGA-PEG/PFC emulsion for highly efficient reoxygenation to cell and organism. HCT 116 cells are used as a model cell, whose viability has a significant enhancement after reoxygenation with PLGA-PEG/PFC emulsion because of the sufficient and timely oxygen supply. Meanwhile, hypoxia-reoxygenation injury will happen along with cell hypoxia-reoxygenation treatment, which is reflected by increasing reactive oxygen species (ROS) in cells. However, the integration of intracellular ROS and cell viability implies that the degree of hypoxia-reoxygenation injury is sublethal to HCT116 cells when the concentration of PLGA-PEG/PFC emulsion is lower than 0.2 mg/mL. Furthermore, the change of the expression level of hypoxia-inducible factor-1伪 (HIF-1伪) is similar to that of cell viability during reoxygenation, which suggests that HIF-1伪 or its downstream proteins may make a significant contribution to cell viability. In vivo oxygen supply is assessed in rats through pulmonary delivery, which shows that PLGA-PEG/PFC emulsion can supply oxygen to rats and improve rats鈥?lung ventilation.