A Retrievable and Highly Selective Fluorescent Probe for Monitoring Sulfide and Imaging in Living Cells

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• 作者：Fengping Hou ; Liang Huang ; Pinxian Xi ; Ju Cheng ; Xuefei Zhao ; Guoqiang Xie ; Yanjun Shi ; Fengjuan Cheng ; Xiaojun Yao ; Decheng Bai ; Zhengzhi Zeng
• 刊名：Inorganic Chemistry
• 出版年：2012
• 出版时间：February 20, 2012
• 年：2012
• 卷：51
• 期：4
• 页码：2454-2460
• 全文大小：491K
• 年卷期：v.51,no.4(February 20, 2012)
• ISSN：1520-510X

文摘

A novel selective fluorescent chemosensor based on an 8-hydroxyquinoline-appended fluorescein derivative (L1) was synthesized and characterized. Once combined with Cu²⁺, it displayed high specificity for sulfide anion. Among the various anions, only sulfide anion induced the revival of fluoresecence of L1, which was quenched by Cu²⁺, resulting in 鈥渙ff鈥搊n鈥?type sensing of sulfide anion. What鈥檚 more, the sensor was retrievable to indicate sulfide anions with Cu²⁺, and S^{2鈥?/sup>, in turn, increased. With the addition of Cu2+, compound L1 could give rise to a visible pink-to-yellow color change and green fluorescence quenching. The resulting yellow solution could change to pink and regenerate to green fluorescence immediately upon the addition of sulfide anion; however, no changes were observed in the presence of other anions, including CN鈥?/sup>, P₂O₇4鈥?/sup>, and other forms of sulfate, making compound L1 an extremely selective and efficient sulfide chemosensor. The signal transduction occurs via reversible formation鈥搒eparation of complex L1Cu and CuS. What鈥檚 more, the biological imaging study has demonstrated that the chemosensor can detect sulfur anions in biological systems at a relatively low concentration.}