DNA Separation in Nanowall Array Chips

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• 作者：Takao Yasui ; Noritada Kaji ; Ryo Ogawa ; Shingi Hashioka ; Manabu Tokeshi ; Yasuhiro Horiike ; Yoshinobu Baba
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：September 1, 2011
• 年：2011
• 卷：83
• 期：17
• 页码：6635-6640
• 全文大小：895K
• 年卷期：v.83,no.17(September 1, 2011)
• ISSN：1520-6882

文摘

A nanowall array structure was fabricated on a quartz chip as a separation matrix of DNA fragments, and a 30 s separation was realized for a mixture of DNA fragments (48.5 and 1 kbp fragments) by applying the electric voltage. A longer DNA fragment migrates faster than a shorter one in a nanowall array chip, and it is completely different from the separation of DNA based on gel electrophoresis, nanopillar chips, and nanoparticle array chips. Although the result is similar to DNA separation by entropic trapping, it could not be fully explained by entropic trapping phenomena. Direct observation of single-DNA molecular dynamics inside a nanowall array structure indicates that both confined elongation and relaxation recoiling of a DNA molecule occur, and an elongated DNA molecule migrates faster than a recoiled DNA molecule. Numerical fitting of DNA molecular dynamics reveals that the balance between times for the transverse of a DNA molecule in the nanowall array chip and the relaxation-recoiling of a DNA molecule governs the separation of DNA.