A Single CH/ Weak Hydrogen Bond Governs Stability and the Photocycle of the Photoactive Yellow Protein
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- 作者：Miki Harigai ; Mikio Kataoka ; Yasushi Imamoto
- 刊名：Journal of the American Chemical Society
- 出版年：2006
- 出版时间：August 23, 2006
- 年：2006
- 卷：128
- 期：33
- 页码：10646 - 10647
- 全文大小：114K
- 年卷期：v.128,no.33(August 23, 2006)
- ISSN：1520-5126
文摘
Importance of the CH/
interaction on the structure and function of the photoactive yellow protein (PYP) was substantiated. Focusing on the phenyl ring of Phe6 adjacent to the alkyl chain of Lys123, the mutants for these amino acid residues were characterized. The results demonstrated that the mutants lacking the -electron at position 6 or the alkyl chain at position 123 show substantial malfunction. This is a clear example that single CH/ weak interaction plays a crucial role in the normal action of the protein.
interaction on the structure and function of the photoactive yellow protein (PYP) was substantiated. Focusing on the phenyl ring of Phe6 adjacent to the alkyl chain of Lys123, the mutants for these amino acid residues were characterized. The results demonstrated that the mutants lacking the -electron at position 6 or the alkyl chain at position 123 show substantial malfunction. This is a clear example that single CH/ weak interaction plays a crucial role in the normal action of the protein.