Fluorescence-Based Detection of Point Mutation in DNA Sequences by CdS Quantum Dot Aggregation

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• 作者：Taehoon Kim ; Minho Noh ; Hosub Lee ; Sang-Woo Joo ; So Yeong Lee ; Kangtaek Lee
• 刊名：Journal of Physical Chemistry B
• 出版年：2009
• 出版时间：October 29, 2009
• 年：2009
• 卷：113
• 期：43
• 页码：14487-14490
• 全文大小：202K
• 年卷期：v.113,no.43(October 29, 2009)
• ISSN：1520-5207

文摘

We present a novel method for the detection of single base mismatch based on fluorescence quenching that unmodified CdS quantum dots exhibit upon aggregation. Target DNA sequences of interest are breast cancer 2 (BRCA2) and signal-induced proliferation-associated gene 1 (Sipa1) sequences. We monitor aggregation of CdS quantum dots upon addition of double-stranded DNAs at different salt concentration using quasi-elastic light scattering (QELS), transmission electron microscopy (TEM), photoluminescence spectroscopy, and zeta potential measurement. Our results indicate that the double-stranded DNA with a perfectly matched sequence can easily be discerned by naked eye from the single base mismatched one due to the fluorescence quenching phenomenon caused by selective aggregation of the CdS quantum dots.