Optically Encoded Multifunctional Nanospheres for One-Pot Separation and Detection of Multiplex DNA Sequences

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• 作者：Jun Hu ; Cong-Ying Wen ; Zhi-Ling Zhang ; Min Xie ; Jiao Hu ; Min Wu ; Dai-Wen Pang
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：December 17, 2013
• 年：2013
• 卷：85
• 期：24
• 页码：11929-11935
• 全文大小：422K
• 年卷期：v.85,no.24(December 17, 2013)
• ISSN：1520-6882

文摘

In this study, we report a simple method for simultaneous detection of multiplex DNA sequences, including complementary DNA (cDNA) sequences of HIV and HCV, DNA sequence of HBV, with QDs-encoded fluorescent nanospheres and nano-纬-Fe₂O₃-coated magnetic nanospheres. Detection was achieved on a fluorescence spectrophotometer without additional auxiliary instruments, and the detection limit was about 100 pM. Here, QDs-encoded fluorescent nanospheres (FNS) with different photoluminescent properties, and magnetic nanospheres (MNS) were separately fabricated by stepwise assembly of hydrophobic QDs or nano-纬-Fe₂O₃ on the surface of branched poly(ethylene imine) (PEI)-coated nanospheres in precisely controlled amounts, finally followed by silica encapsulation. FNS-labeled probe DNAs and MNS-labeled capture DNAs were used to hybridize with the corresponding targets at the same time. After magnetic separation, the sandwich-structured adducts were measured by fluorescence spectrophotometry. The results indicated that the targets could be detected with high sensitivity. This method is convenient, fast enough, and capable of high anti-interference. Therefore, it is expected to be used for simultaneous detection and separation of multiple targets at high levels of purity and throughput.