Fluorescence Blinking as an Output Signal for Biosensing

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• 作者：Brandon Roark ; Jenna A. Tan ; Anna Ivanina ; Morgan Chandler ; Jose Castaneda ; Ho Shin Kim ; Shriram Jawahar ; Mathias Viard ; Strahinja Talic ; Kristin L. Wustholz ; Yaroslava G. Yingling ; Marcus Jones ; Kirill A. Afonin
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：November 23, 2016
• 年：2016
• 卷：1
• 期：11
• 页码：1295-1300
• 全文大小：577K
• ISSN：2379-3694

文摘

We demonstrate the first biosensing strategy that relies on quantum dot (QD) fluorescence blinking to report the presence of a target molecule. Unlike other biosensors that utilize QDs, our method does not require the analyte to induce any fluorescence intensity or color changes, making it readily applicable to a wide range of target species. Instead, our approach relies on the understanding that blinking, a single particle phenomenon, is obscured when several QDs lie within the detection volume of a confocal microscope. If QDs are engineered to aggregate when they encounter a particular target molecule, the observation of quasi-continuous emission should indicate its presence. As proof of concept, we programmed DNAs to drive rapid isothermal assembly of QDs in the presence of a target strand (oncogene K-ras). The assemblies, confirmed by various gel techniques, contained multiple QDs and were readily distinguished from free QDs by the absence of blinking.