Accumulation and Localization of Cesium in Edible Mushroom (Pleurotus ostreatus) Mycelia

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• 作者：Hideo Sugiyama ; Mitsuko N. Takahashi ; Hiroshi Terada ; Chikako Kuwahara ; Chika Maeda ; Yojiro Anzai ; Fumio Kato
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：October 22, 2008
• 年：2008
• 卷：56
• 期：20
• 页码：9641-9646
• 全文大小：255K
• 年卷期：v.56,no.20(October 22, 2008)
• ISSN：1520-5118

文摘

The characteristics of Cs accumulation and localization in edible mushrooms were examined using the mycelia of Pleurotus ostreatus-Y1. Scanning electron microscope images revealed the existence of white spots, and energy dispersive X-ray microanalyzer analysis indicated the presence of larger amounts of Cs and P in these spots in mycelia cultured on medium containing 25 mM CsCl. The ¹³⁷Cs activities in the mycelia were approximately 4−6 times higher than those in water used for ¹³⁷Cs elution. Higher Cs concentrations in the sediment fraction including vacuolar pellets were obtained compared to the upper fractions. It was observed that yellowish spots caused by the fluorescence of 4′,6-diamidino-2-phenylindole (DAPI)-stained polyphosphate were localized in the mycelia. The higher fluorescence intensity of the yellowish-grained spots was measured in comparison with other regions in the mycelium. These results suggested that Cs in the mycelia was trapped by polyphosphate in vacuoles or other organelles.