Development of Phage Immuno-Loop-Mediated Isothermal Amplification Assays for Organophosphorus Pesticides in Agro-products

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• 作者：Xiude Hua ; Wei Yin ; Haiyan Shi ; Ming Li ; Yanru Wang ; Hong Wang ; Yonghao Ye ; Hee Joo Kim ; Shirley J. Gee ; Minghua Wang ; Fengquan Liu ; Bruce D. Hammock
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：August 19, 2014
• 年：2014
• 卷：86
• 期：16
• 页码：8441-8447
• 全文大小：361K
• ISSN：1520-6882

文摘

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL^鈥?. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.