PDZ-Reactive Peptide Activates Ephrin-B Reverse Signaling and Inhibits Neuronal Chemotaxis

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• 作者：Yongsheng Yu ; Miao Liu ; Tsz Tsun Ng ; Feng Huang ; Yunyu Nie ; Rui Wang ; Zhong-Ping Yao ; Zigang Li ; Jiang Xia
• 刊名：ACS Chemical Biology
• 出版年：2016
• 出版时间：January 15, 2016
• 年：2016
• 卷：11
• 期：1
• 页码：149-158
• 全文大小：564K
• ISSN：1554-8937

文摘

Intracellular reactions on nonenzymatic proteins that activate cellular signals are rarely found. We report one example here that a designed peptide derivative undergoes a nucleophilic reaction specifically with a cytosolic PDZ protein inside cells. This reaction led to the activation of ephrin-B reverse signaling, which subsequently inhibited SDF-1 induced neuronal chemotaxis of human neuroblastoma cells and mouse cerebellar granule neurons. Our work provides direct evidence that PDZ-RGS3 bridges ephrin-B reverse signaling and SDF-1 induced G protein signaling for the first time.