Capillary-Channeled Polymer Fibers as a Stationary Phase for Desalting of Protein Solutions for Electrospray Ionization Mass Spectrometry Analysis

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• 作者：D. Seth Fornea ; Yonnie Wu ; R. Kenneth Marcus
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：August 1, 2006
• 年：2006
• 卷：78
• 期：15
• 页码：5617 - 5621
• 全文大小：233K
• 年卷期：v.78,no.15(August 1, 2006)
• ISSN：1520-6882

文摘

Micropipet solid-phase extraction (SPE) tips have beenused to desalt and purify proteins and peptides frommixtures of buffers and biological solutions. Removingsalts and buffers prior to electrospray ionization massspectrometry (ESI-MS) characterization improves thedetection limits and the sensitivity of the protein analyses.Recently, capillary-channeled polymer (C-CP) fibers havebeen investigated as stationary phases for high-performance liquid chromatography separations of proteins.Polypropylene C-CP fibers incorporated as sorbent materials in micro-SPE tips are shown to effectively removeboth inorganic and organic buffers from proteins indefined solutions. The architecture of the fibers provideslarge surface areas in comparison to conventional roundfibers and is readily packed into capillaries that can beaffixed to micropipet tips. Desalting of protein solutionsis demonstrated for ESI-MS analysis through increasedsignal-to-noise ratios and reduced spectral complexity.