Oleifolioside A Mediates Caspase-Independent Human Cervical Carcinoma HeLa Cell Apoptosis Involving Nuclear Relocation of Mitochondrial Apoptogenic Factors AIF and EndoG

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• 作者：Hai Yang Yu ; Cheng-Yun Jin ; Kyoung-Sook Kim ; Young-Choon Lee ; Shin-Hyung Park ; Gi-Young Kim ; Wun-Jae Kim ; Hyung-In Moon ; Yung Hyun Choi ; Jai-Heon Lee
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：May 30, 2012
• 年：2012
• 卷：60
• 期：21
• 页码：5400-5406
• 全文大小：357K
• 年卷期：v.60,no.21(May 30, 2012)
• ISSN：1520-5118

文摘

Apoptosis, the main type of programmed cell death, plays an essential role in a variety of biological events. Whereas 鈥渃lassical鈥?apoptosis is dependent on caspase activation, caspase-independent death is increasingly recognized as an alternative pathway. To develop new anticancer agents, oleifolioside A was isolated from Dendropanax morbifera Leveille and the biochemical mechanisms of oleifolioside A-induced apoptosis in HeLa cells were investigated. Exposure to oleifolioside A resulted in caspase activation and typical features of apoptosis, although cell death was not prevented by caspase inhibition. Oleifolioside A treatment induced up-regulation of Bad, loss of mitochondrial membrane potential, nuclear relocation of mitochondrial factors, apoptosis-inducing factor (AIF), endonuclease G (EndoG), and apoptosis induction. This is the first report of anticancer activity of oleifolioside A, and nuclear translocation of AIF and EndoG in oleifolioside A-treated HeLa cells might represent an alternative death signaling pathway in the absence of caspase activity.

Keywords:

oleifolioside A; apoptosis; AIF; EndoG; HeLa