Inhibition of A2780 Human Ovarian Carcinoma Cell Proliferation by a Rubus Component, Sanguiin H-6
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- 作者：Dahae Lee ; Hyeonseok Ko ; Young-Joo Kim ; Su-Nam Kim ; Kyung-Chul Choi ; Noriko Yamabe ; Ki Hyun Kim ; Ki Sung Kang ; Hyun Young Kim ; Takayuki Shibamoto
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2016
- 出版时间：February 3, 2016
- 年：2016
- 卷：64
- 期：4
- 页码：801-805
- 全文大小：369K
- ISSN：1520-5118
文摘
The effects of a red raspberry component, sanguiin H-6 (SH-6), on the induction of apoptosis and the related signaling pathways in A2780 human ovarian carcinoma cells were investigated. SH-6 caused an antiproliferative effect and a severe morphological change resembling that of apoptotic cell death but no effect on the cancer cell cycle arrest. In addition, SH-6 induced an early apoptotic effect and activation of caspases as well as the cleavage of PARP, which is a hallmark of apoptosis. The early apoptotic percentages of A2780 cells exposed to 20 and 40 μM SH-6 were 35.39 and 41.76, respectively. Also, SH-6 caused the activation of mitogen-activated protein kinases (MAPKs), especially p38, and the increase of truncated p15/BID. These results in the present study suggest that the apoptosis of A2780 human ovarian carcinoma cells by SH-6 is mediated by the MAPK p38 and a caspase-8-dependent BID cleavage pathway.