Metabolomics Revealed Novel Isoflavones and Optimal Cultivation Time of Cordyceps militaris Fermentation

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• 作者：Jung Nam Choi ; Jiyoung Kim ; Mi Yeon Lee ; Dong Ki Park ; Young-Shick Hong ; Choong Hwan Lee
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：April 14, 2010
• 年：2010
• 卷：58
• 期：7
• 页码：4258-4267
• 全文大小：1043K
• 年卷期：v.58,no.7(April 14, 2010)
• ISSN：1520-5118

文摘

Germinated soybean (GS) cultivated with Cordyceps militaris (GSC) might be a promising efficacious source of novel bioactive compounds. In this study, the metabolome changes between GS and GSC were investigated by liquid chromatography−mass spectrometry (LC-MS) and gas chromatography−mass spectrometry (GC-MS) analysis coupled with a multivariate data set. Principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) of GSC clearly showed higher levels of soyasaponin Bd, soyasaponin Bc(II), daidzein, genistein, four isoflavones (compounds 1−4), glycerol, proline, glutamine, pentitol, fructose, inositol, octadecanoic acid, and sucrose together with lower levels of pyroglutamic acid, citric acid, histidine, and palmitic acid in GSC than in GS. The structures of compounds 1−4 were analyzed by mass and NMR spectroscopy and were determined to be novel isoflavone methyl-glycosides (daidzein 7-O-β-d-glucoside 4′′-O-methylate (1), glycitein 7-O-β-d-glucoside 4′′-O-methylate (2), genistein 7-O-β-d-glucoside 4′′-O-methylate (3), and genistein 4′-O-β-d-glucoside 4′′-O-methylate (4)). Multivariate statistical models showed that metabolic changes of GSC were maximal within 1 week after the C. militaris inoculation, consistent with the strongest antioxidant activity of GSC cultivated for 1 week. This metabolomics study provides valuable information in regard to optimizing the cultivation process for bioactive compound production and describes an efficient way to screen for novel bioactive compounds from GSC.