Photonic Gene Circuits by Optically Addressable siRNA-Au Nanoantennas

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• 作者：Somin Eunice Lee ; Darryl Y. Sasaki ; Younggeun Park ; Ren Xu ; James S. Brennan ; Mina J. Bissell ; Luke P. Lee
• 刊名：ACS Nano
• 出版年：2012
• 出版时间：September 25, 2012
• 年：2012
• 卷：6
• 期：9
• 页码：7770-7780
• 全文大小：620K
• 年卷期：v.6,no.9(September 25, 2012)
• ISSN：1936-086X

文摘

The precise perturbation of gene circuits and the direct observation of signaling pathways in living cells are essential for both fundamental biology and translational medicine. Current optogenetic technology offers a new paradigm of optical control for cells; however, this technology relies on permanent genomic modifications with light-responsive genes, thus limiting dynamic reconfiguration of gene circuits. Here, we report precise control of perturbation and reconfiguration of gene circuits in living cells by optically addressable siRNA-Au nanoantennas. The siRNA-Au nanoantennas fulfill dual functions as selectively addressable optical receivers and biomolecular emitters of small interfering RNA (siRNA). Using siRNA-Au nanoantennas as optical inputs to existing circuit connections, photonic gene circuits are constructed in living cells. We show that photonic gene circuits are modular, enabling subcircuits to be combined on-demand. Photonic gene circuits open new avenues for engineering functional gene circuits useful for fundamental bioscience, bioengineering, and medical applications.

Keywords:

siRNA; RNA interference; optogenetics; plasmonics; gene delivery; gene therapy; gold nanorod