Complementary Approaches To Gauge the Bioavailability and Distribution of Ingested Berry Polyphenolics
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- 作者：Mary Ann Lila ; David M. Ribnicky ; Leonel E. Rojo ; Patricio Rojas-Silva ; Andrew Oren ; Robert Havenaar ; Elsa M. Janle ; Ilya Raskin ; Gad G. Yousef ; Mary H. Grace
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：June 13, 2012
- 年：2012
- 卷：60
- 期：23
- 页码：5763-5771
- 全文大小：360K
- 年卷期：v.60,no.23(June 13, 2012)
- ISSN：1520-5118
文摘
Two different strategies for investigating the likely fate, after ingestion, of natural, bioactive berry constituents (anthocyanins and other non-nutritive flavonoids) are compared. A model of the human gastrointestinal tract (TIM-1) that mimicked the biological environment from the point of swallowing and ingestion through the duodenum, jejunum, and ileum (but not the colon) was used to monitor the stability and bioaccessibility of anthocyanins from both maqui berry and wild blueberry. TIM-1 revealed that most anthocyanins were bioaccessible between the second and third hours after intake. Alternatively, biolabeled anthocyanins and other flavonoids generated in vitro from berry and grape cell cultures were administered to in vivo (rodent) models, allowing measurement and tracking of the absorption and transport of berry constituents and clearance through the urinary tract and colon. The advantages and limitations of the alternative strategies are considered.