Highly Sensitive Graphene鈥揚t Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection

详细信息    查看全文

• 作者：Yuanyuan Zhang ; Xiaoyun Bai ; Xuemei Wang ; Kwok-Keung Shiu ; Yanliang Zhu ; Hui Jiang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：October 7, 2014
• 年：2014
• 卷：86
• 期：19
• 页码：9459-9465
• 全文大小：457K
• ISSN：1520-6882

文摘

A sensitive hydrogen peroxide (H₂O₂) sensor was constructed based on graphene鈥揚t (RGO鈥揚t) nanocomposites and used to measure the release of H₂O₂ from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K₂PtCl₆ solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO鈥揚t nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H₂O₂. The relevant detection limit of H₂O₂ is 鈭?.2 渭M with a wide linear range from 0.5 渭M to 3.475 mM, displaying a much higher sensitivity (459 卤 3 mA M^鈥? cm^鈥?, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H₂O₂ release from living cells because of its low detection limit, wide linear range, and higher sensitivity.