Standing Surface Acoustic Wave Based Cell Coculture
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- 作者：Sixing Li ; Feng Guo ; Yuchao Chen ; Xiaoyun Ding ; Peng Li ; Lin Wang ; Craig E. Cameron ; Tony Jun Huang
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：October 7, 2014
- 年：2014
- 卷：86
- 期：19
- 页码：9853-9859
- 全文大小：467K
- ISSN：1520-6882
文摘
Precise reconstruction of heterotypic cell鈥揷ell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell鈥揷ell interactions.