Antihypertensive Properties of Spinach Leaf Protein Digests
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- 作者：Yanjun Yang ; Ewa D. Marczak ; Hachiro Usui ; Yukio Kawamura ; and Masaaki Yoshikawa
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：April 21, 2004
- 年：2004
- 卷：52
- 期：8
- 页码：2223 - 2225
- 全文大小：55K
- 年卷期：v.52,no.8(April 21, 2004)
- ISSN：1520-5118
文摘
Leaf protein containing approximately 50% rubisco (ribulose bisphosphate carboxylase/oxygenase)was obtained from fresh spinach leaf with the use of a simple extraction method. Pepsin and pepsin-pancreatin digests of spinach leaf protein have potent angiotensin-I converting enzyme inhibitoryproperties with IC50 values of 56 and 120 
g/mL, respectively. Both digests of leaf protein haveantihypertensive effects after oral administration to spontaneously hypertensive rats (SHR) withminimum effective doses of 0.25 and 0.5 g/kg, respectively. The maximum antihypertensive effectfor the pepsin digest was observed 4 h after oral administration, while for the pepsin-pancreatindigest, the maximum effect was observed 2 h after oral administration. Undigested spinach leaf proteindid not exert any significant antihypertensive effect after oral administration to SHR at doses of 0.5and 1 g/kg. Obtained results show that the pepsin digest of leaf protein may be useful in treatmentof hypertension.Keywords: Leaf protein isolation; spinach; leaf protein digest; ACE inhibitors; antihypertensive effect;spontaneously hypertensive rat
g/mL, respectively. Both digests of leaf protein haveantihypertensive effects after oral administration to spontaneously hypertensive rats (SHR) withminimum effective doses of 0.25 and 0.5 g/kg, respectively. The maximum antihypertensive effectfor the pepsin digest was observed 4 h after oral administration, while for the pepsin-pancreatindigest, the maximum effect was observed 2 h after oral administration. Undigested spinach leaf proteindid not exert any significant antihypertensive effect after oral administration to SHR at doses of 0.5and 1 g/kg. Obtained results show that the pepsin digest of leaf protein may be useful in treatmentof hypertension.Keywords: Leaf protein isolation; spinach; leaf protein digest; ACE inhibitors; antihypertensive effect;spontaneously hypertensive rat