Phosphorescent Differential Sensing of Physiological Phosphates with Lanthanide Ions-Modified Mn-Doped ZnCdS Quantum Dots

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• 作者：Hengwei He ; Chenghui Li ; Yunfei Tian ; Peng Wu ; Xiandeng Hou
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5892-5897
• 全文大小：475K
• 年卷期：0
• ISSN：1520-6882

文摘

Phosphates, both inorganic and organic, play fundamental roles in numerous biological and chemical processes. The biological functions of phosphates connect with each other, analysis of single phosphate-containing biomolecule therefore cannot reveal the exact biological significance of phosphates. Sensor array is therefore the best choice for differentiation analysis of physiological phosphates. Lanthanide ions possess high affinity toward physiological phosphates, while lanthanide ions can also efficiently quench the luminescence of quantum dots (QDs). Taking lanthanide ions as cartridges, here we proposed a sensor array for sensing of physiological phosphates based on lanthanide ions-modified Mn-doped ZnCdS phosphorescent QDs in the manner of indicator-displacement assay. A series of lanthanide ions were selected as quencher for phosphorescent QDs. Physiological phosphates could subsequently displace the quencher and recover the phosphorescence. Depending on their varied phosphorescence restoration, a sensor array was thus developed. The photophysics of phosphorescence quenching and restoration were studied in detail for better understanding the mechanism of the sensor array. The exact contribution of each sensor element to the sensor array was evaluated. Those sensor elements with little contribution to the differentiation analysis were removed for narrowing the size of the array. The proposed sensor array was successfully explored for probing nucleotide phosphates-involved enzymatic processes and their metabolites, simulated energy charge changes, and analysis of physiological phosphates in biological samples.