Bimodal Imprint Chips for Peptide Screening: Integration of High-Throughput Sequencing by MS and Affinity Analyses by Surface Plasmon Resonance Imaging

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• 作者：Weizhi Wang ; Menglin Li ; Zewen Wei ; Zihua Wang ; Xiangli Bu ; Wenjia Lai ; Shu Yang ; He Gong ; Hui Zheng ; Yuqiao Wang ; Ying Liu ; Qin Li ; Qiaojun Fang ; Zhiyuan Hu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：April 15, 2014
• 年：2014
• 卷：86
• 期：8
• 页码：3703-3707
• 全文大小：390K
• 年卷期：v.86,no.8(April 15, 2014)
• ISSN：1520-6882

文摘

Peptide probes and drugs have widespread applications in disease diagnostics and therapy. The demand for peptides ligands with high affinity and high specificity toward various targets has surged in the biomedical field in recent years. The traditional peptide screening procedure involves selection, sequencing, and characterization steps, and each step is manual and tedious. Herein, we developed a bimodal imprint microarray system to embrace the whole peptide screening process. Silver-sputtered silicon chip fabricated with microwell array can trap and pattern the candidate peptide beads in a one-well-one-bead manner. Peptides on beads were photocleaved in situ. A portion of the peptide in each well was transferred to a gold-coated chip to print the peptide array for high-throughput affinity analyses by surface plasmon resonance imaging (SPRi), and the peptide left in the silver-sputtered chip was ready for in situ single bead sequencing by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Using the bimodal imprint chip system, affinity peptides toward AHA were efficiently screened out from the 7 脳 10⁴ peptide library. The method provides a solution for high efficiency peptide screening.