Effect of Different Fertilizers on Nitrogen Isotope Composition and Nitrate Content of Brassica campestris

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• 作者：Yuwei Yuan ; Ming Zhao ; Zhiheng Zhang ; Tianjin Chen ; Guiling Yang ; Qiang Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：February 15, 2012
• 年：2012
• 卷：60
• 期：6
• 页码：1456-1460
• 全文大小：197K
• 年卷期：v.60,no.6(February 15, 2012)
• ISSN：1520-5118

文摘

The effect of different fertilizers on the 未¹⁵N value, nitrate concentration, and nitrate reductase activity of Brassica campestris and the 未¹⁵N value of soil has been investigated through a pot experiment. The 未¹⁵N mean value of B. campestris at the seedling stage observed in the composted chicken treatment (+8.65鈥? was higher than that of chemical fertilizer treatment (+5.73鈥?, compost-chemical fertilizer (+7.53鈥?, and control check treatment (+7.86鈥?. There were significantly different 未¹⁵N values (p < 0.05) between B. campestris cultivated with composted chicken manure treatment and with chemical fertilizer treatment. The similar results were also found at the middle stage and the terminal stage. The variation of 未¹⁵N value in soil for different treatments was smaller than that of B. campestris, which was +6.71鈥?8.12鈥? +6.83鈥?8.24鈥? and +6.85鈥?.4鈥? respectively, at seedling stage, middle stage, and terminal stage. With the growth of B. campestris, the nitrate content decreased in all treatments, and the nitrate reductase activity in B. campestris increased except for the CK. Results suggested that the 未¹⁵N values of B. campestris and soil were more effected by the fertilizer than by the dose level, and the 未¹⁵N value analysis could be used as a tool to discriminate the B. campestris cultivated with composted manure or chemical fertilizer.

Keywords:

stable nitrogen isotope; fertilizer; nitrate; nitrate reductase; Brassica campestris