Single-Molecule Force Spectroscopy of an Artificial DNA Duplex Comprising a Silver(I)-Mediated Base Pair

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• 作者：Xinxin Tan ; Stefanie Litau ; Xi Zhang ; Jens M眉ller
• 刊名：Langmuir
• 出版年：2015
• 出版时间：October 20, 2015
• 年：2015
• 卷：31
• 期：41
• 页码：11305-11310
• 全文大小：397K
• ISSN：1520-5827

文摘

Single-molecule force spectroscopy measurements of a DNA duplex comprising an artificial metal-mediated base pair are reported. The measurements reveal that DNA duplexes comprising one central imidazole:imidazole mispair rupture at lower forces than a related duplex with canonical base pairs only. In contrast, DNA duplexes with one central imidazole鈥揂g⁺鈥搃midazole base pair (formed by the addition of Ag⁺ to the aforementioned duplex with the mispair) rupture at higher forces. These measurements indicate for the first time that the increase in thermal stability of a nucleic acid duplex that is observed upon the formation of a metal-mediated base pair is accompanied by a concomitant mechanical stabilization. In fact, the mechanical stabilization even exceeds the thermal one. This result indicates that nucleic acids with metal-mediated base pairs should be ideal building blocks for rigid functionalized DNA nano-objects.