Effects of d-Pinitol on Insulin Resistance through the PI3K/Akt Signaling Pathway in Type 2 Diabetes Mellitus Rats

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• 作者：Yunfeng Gao ; Mengna Zhang ; Tianchen Wu ; Mengying Xu ; Haonan Cai ; Zesheng Zhang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 8, 2015
• 年：2015
• 卷：63
• 期：26
• 页码：6019-6026
• 全文大小：461K
• ISSN：1520-5118

文摘

d-Pinitol, a compound isolated from Pinaceae and Leguminosae plants, has been reported to possess insulin-like properties. Although the hypoglycemic activity of d-pinitol was recognized in recent years, the molecular mechanism of d-pinitol in the treatment of diabetes mellitus remains unclear. In this investigation, a model of type 2 diabetes mellitus (T2DM) with insulin resistance was established by feeding a high-fat diet (HFD) and injecting streptozocin (STZ) to Sprague鈥揇awley (SD) rats, targeting the exploration of more details of the mechanism in the therapy of T2DM. d-Pinitol was administrated to the diabetic rats as two doses [30, 60 mg/(kg路body weight路day)]. The level of fasting blood glucose (FBG) was decreased 12.63% in the high-dosage group, and the ability of oral glucose tolerance was improved in d-pinitol-treated groups. The biochemical indices revealed that d-pinitol had a positive effect on hypoglycemic activity. Western boltting suggested that d-pinitol could promote the expression of the phosphatidylinositol-3-kinase (PI3K) p85, PI3Kp110, as well as the downstream target protein kinase B/Akt (at Ser473). Besides, d-pinitol inhibited the expression of glycogen synthesis kinase-3尾 (GSK-3尾) protein and regulated the expression of glycogen synthesis (GS) protein and then accelerated the glycogen synthesis. Above all, d-pinitol played a positive role in regulating insulin-mediated glucose uptake in the liver through translocation and activation of the PI3K/Akt signaling pathway in T2DM rats.