Graphene Oxide Attenuates the Cytotoxicity and Mutagenicity of PCB 52 via Activation of Genuine Autophagy

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• 作者：Yun Liu ; Xinan Wang ; Juan Wang ; Yaguang Nie ; Hua Du ; Hui Dai ; Jingjing Wang ; Mudi Wang ; Shaopeng Chen ; Tom K. Hei ; Zhaoxiang Deng ; Lijun Wu ; An Xu
• 刊名：Environmental Science & Technology
• 出版年：2016
• 出版时间：March 15, 2016
• 年：2016
• 卷：50
• 期：6
• 页码：3154-3164
• 全文大小：588K
• ISSN：1520-5851

文摘

Graphene oxide (GO), owing to its large surface area and abundance of oxygen-containing functional groups, is emerging as a potential adsorbent for polychlorinated biphenyls (PCBs), which accumulate over time and are harmful to both natural ecosystems and human health. However, the effect of GO against PCB-induced toxicity remains largely unexplored. The present study aimed to investigate the protective effect of GO against PCB 52 induced cytotoxic and genotoxic response in mammalian cells at various exposure conditions and clarify the protective role of autophagy. Pretreatment with GO dramatically decreased PCB 52 induced cytotoxicity and CD59 gene mutation in human–hamster hybrid (A_L) cells. The toxic response in cells either pretreated with PCB 52 and then treated with GO or concurrently treated with GO and PCB 52 did not differ significantly from the toxic response in the cells treated with PCB 52 alone. Using autophagy inhibitors (3-methyladenine and wortmannin) and inducers (trehalose and rapamycin), we found that genuine autophagy induced by GO was involved in decreasing PCB 52 induced toxicity. These findings suggested that GO has an antagonistic effect against the toxicity of PCB 52 mainly by triggering a genuine autophagic process, which might provide new insights into the potential application of GO in PCB disposal and environmental and health risk assessment.