Monitoring Diet Effects via Biofluids and Their Implications for Metabolomics Studies

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• 作者：Haiwei Gu ; Huanwen Chen ; Zhengzheng Pan ; Ayanna U. Jackson ; Nari Talaty ; Bowei Xi ; Candice Kissinger ; Chester Duda ; Doug Mann ; Daniel Raftery ; R. Graham Cooks
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：January 1, 2007
• 年：2007
• 卷：79
• 期：1
• 页码：89 - 97
• 全文大小：271K
• 年卷期：v.79,no.1(January 1, 2007)
• ISSN：1520-6882

文摘

The effect of diet on metabolites found in rat urinesamples has been investigated using nuclear magneticresonance (NMR) and a new ambient ionization massspectrometry experiment, extractive electrospray ionization mass spectrometry (EESI-MS). Urine samples fromrats with three different dietary regimens were readilydistinguished using multivariate statistical analysis onmetabolites detected by NMR and MS. To observe theeffect of diet on metabolic pathways, metabolites relatedto specific pathways were also investigated using multivariate statistical analysis. Discrimination is increased bymaking observations on restricted compound sets. Changesin diet at 24-h intervals led to predictable changes in thespectral data. Principal component analysis was used toseparate the rats into groups according to their differentdietary regimens using the full NMR, EESI-MS data orrestricted sets of peaks in the mass spectra correspondingonly to metabolites found in the urea cycle and metabolism of amino groups pathway. By contrast, multivariateanalysis of variance from the score plots showed thatmetabolites of purine metabolism obscure the classification relative to the full metabolite set. These resultssuggest that it may be possible to reduce the number ofstatistical variables used by monitoring the biochemicalvariability of particular pathways. It should also be possible by this procedure to reduce the effect of diet in thebiofluid samples for such purposes as disease detection.