A Cell-Surface-Anchored Ratiometric Fluorescent Probe for Extracellular pH Sensing
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- 作者：Guoliang Ke ; Zhi Zhu ; Wei Wang ; Yuan Zou ; Zhichao Guan ; Shasha Jia ; Huimin Zhang ; Xuemeng Wu ; Chaoyong James Yang
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2014
- 出版时间：September 10, 2014
- 年：2014
- 卷：6
- 期：17
- 页码：15329-15334
- 全文大小：386K
- ISSN：1944-8252
文摘
Accurate sensing of the extracellular pH is a very important yet challenging task in biological and clinical applications. This paper describes the development of an amphiphilic lipid鈥揇NA molecule as a simple yet useful cell-surface-anchored ratiometric fluorescent probe for extracellular pH sensing. The lipid鈥揇NA probe, which consists of a hydrophobic diacyllipid tail and a hydrophilic DNA strand, is modified with two fluorescent dyes; one is pH-sensitive as pH indicator and the other is pH-insensitive as an internal reference. The lipid鈥揇NA probe showed sensitive and reversible response to pH change in the range of 6.0鈥?.0, which is suitable for most extracellular studies. In addition, based on simple hydrophobic interactions with the cell membrane, the lipid鈥揇NA probe can be easily anchored on the cell surface with negligible cytotoxicity, excellent stability, and unique ratiometric readout, thus ensuring its accurate sensing of extracellular pH. Finally, this lipid鈥揇NA-based ratiometric pH indicator was successfully used for extracellular pH sensing of cells in 3D culture environment, demonstrating the potential applications of the sensor in biological and medical studies.