Tumor-Penetrating Peptide-Modified DNA Tetrahedron for Targeting Drug Delivery

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• 作者：Zhiwei Xia ; Ping Wang ; Xunwei Liu ; Ting Liu ; Yinan Yan ; Juan Yan ; Jian Zhong ; Gang Sun ; Dannong He
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：March 8, 2016
• 年：2016
• 卷：55
• 期：9
• 页码：1326-1331
• 全文大小：383K
• ISSN：1520-4995

文摘

DNA self-assembling nanostructure has been considered as a promising candidate as a drug delivery vehicle because of its compactness, mechanical stability, and noncytotoxicity. In this work, we developed functional, multiform DNA nanostructures by appending a tumor-penetrating peptide to tetrahedral DNA nanostructure (p-TDN). This functional structure is able to efficiently increase the rate of uptake of glioblastoma cell U87MG compared with the DNA tetrahedron and the double-stranded DNA structures. We found that the DNA tetrahedron plays the main role in the endocytosis of U87MG cells, whereas the tumor-penetrating peptide could also bind to transmembrane glycoprotein neuropilin-1 and mediate the endocytosis of the p-TDN nanostructure. Moreover, given the high efficiency of the growth inhibitory effect of the p-TDN loading doxorubicin hydrochloride, the p-TDN distinguishes itself as a promising candidate as an effective delivery carrier.