Monitoring Molecular Beacon/DNA Interactions Using Atomic Force Microscopy

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• 作者：Yan Jin ; Kemin Wang ; Weihong Tan ; Ping Wu ; Qing Wang ; Hongmei Huang ; Shasheng Huang ; Zhiwen Tang ; and Qiuping Guo
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：October 1, 2004
• 年：2004
• 卷：76
• 期：19
• 页码：5721 - 5725
• 全文大小：70K
• 年卷期：v.76,no.19(October 1, 2004)
• ISSN：1520-6882

文摘

The molecu1ar beacon (MB) is a new fluorescence probecontaining a single-stranded oligonucleotide with a probesequence embedded in complementary sequences thatform a hairpin stem. Due to the inherent fluorescent signaltransduction mechanism, an MB functions as a sensitiveprobe with a high signal-to-background ratio for real-timemonitoring and provides a variety of exciting opportunitiesin DNA, RNA, and protein studies. To better understandthe properties of MBs, the specific interactions betweenMB and target DNA (complementary and one-base mismatch) have been directly investigated by atomic forcemicroscopy. The interaction force between a linear DNAprobe and the target DNA was also detected and compared to that between MB and target DNA. The resultsdemonstrate the high specificity of the MB/target DNAcompared to the linear DNA/target DNA interaction.