A New Nanobiocatalytic System Based on Allosteric Effect with Dramatically Enhanced Enzymatic Performance

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• 作者：Liang-Bing Wang ; You-Cheng Wang ; Rong He ; Awei Zhuang ; Xiaoping Wang ; Jie Zeng ; J. G. Hou
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：January 30, 2013
• 年：2013
• 卷：135
• 期：4
• 页码：1272-1275
• 全文大小：233K
• 年卷期：v.135,no.4(January 30, 2013)
• ISSN：1520-5126

文摘

We report a rational design of CaHPO₄-伪-amylase hybrid nanobiocatalytic system based on allosteric effect and an explanation of the increase in catalytic activity when certain enzymes are immobilized in specific nanomaterials. Employing a calcification approach in aqueous solutions, we acquired such new nanobiocatalytic systems with three different morphologies, i.e., nanoflowers, nanoplates, and parallel hexahedrons. Through studying enzymatic performance of these systems and free 伪-amylase with/without Ca²⁺, we demonstrated how two factors, allosteric regulation and morphology of the as-synthesized nanostructures, predominantly influence enzymatic activity. Benefiting from both the allosteric modulation and its hierarchical structure, CaHPO₄-伪-amylase hybrid nanoflowers exhibited dramatically enhanced enzymatic activity. As a bonus, the new system we devised was found to enjoy higher stability and durability than free 伪-amylase plus Ca²⁺.