Simple Whole-Cell Biodetection and Bioremediation of Heavy Metals Based on an Engineered Lead-Specific Operon

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• 作者：Wei Wei ; Xiangzhi Liu ; Peiqing Sun ; Xin Wang ; Hong Zhu ; Mei Hong ; Zong-Wan Mao ; Jing Zhao
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：March 18, 2014
• 年：2014
• 卷：48
• 期：6
• 页码：3363-3371
• 全文大小：515K
• 年卷期：v.48,no.6(March 18, 2014)
• ISSN：1520-5851

文摘

A lead-specific binding protein, PbrR, and promoter pbr from the lead resistance operon, pbr, of Cupriavidus metallidurans CH34 was incorporated into E. coli in conjunction with an engineered downstream RFP (red fluorescence protein), which allowed for highly sensitive and selective whole-cell detection of lead ions. The subsequent display of PbrR on the E. coli cell surface permitted selective adsorption of lead ions from solution containing various heavy metal ions. The surface-engineered E. coli bacteria effectively protected Arabidopsis thaliana seed germination from the toxicity of lead ions at high concentrations. Engineering the E. coli bacteria harboring these lead-specific elements from the pbr operon may potentially be a valuable general strategy for biodetection and bioremediation of toxic heavy metal ions in the environment.