De Novo Design of a Single-Chain Diphenylporphyrin Metalloprotein
详细信息 查看全文
- 作者：Gretchen M. Bender ; Andreas Lehmann ; Hongling Zou ; Hong Cheng ; H. Christopher Fry ; Don Engel ; Michael J. Therien ; J. Kent Blasie ; Heinrich Roder ; Jeffrey G. Saven ; William F. DeGrado
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：September 5, 2007
- 年：2007
- 卷：129
- 期：35
- 页码：10732 - 10740
- 全文大小：454K
- 年卷期：v.129,no.35(September 5, 2007)
- ISSN：1520-5126
文摘
We describe the computational design of a single-chain four-helix bundle that noncovalentlyself-assembles with fully synthetic non-natural porphyrin cofactors. With this strategy, both the electronicstructure of the cofactor as well as its protein environment may be varied to explore and modulate thefunctional and photophysical properties of the assembly. Solution characterization (NMR, UV-vis) of theprotein showed that it bound with high specificity to the desired cofactors, suggesting that a uniquelystructured protein and well-defined site had indeed been created. This provides a genetically expressedsingle-chain protein scaffold that will allow highly facile, flexible, and asymmetric variations to enable selectiveincorporation of different cofactors, surface-immobilization, and introduction of spectroscopic probes.