Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically

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• 作者：Yali Wang ; Minbo Liu ; Liqi Xie ; Caiyun Fang ; Huanming Xiong ; Haojie Lu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：February 18, 2014
• 年：2014
• 卷：86
• 期：4
• 页码：2057-2064
• 全文大小：367K
• ISSN：1520-6882

文摘

We invented a new method for highly efficient and specific enrichment of glycopeptides using two different nanomaterials synergistically. One is boronic-acid-functionalized Fe₃O₄ nanoparticles, enriching glycopeptides through formation of cyclic boronate esters between the boronic acid groups and the cis鈥揹iol groups on glycopeptides. The other nanomaterial is conventional poly(methyl methacrylate) nanobeads, which have strong adsorption toward nonglycopeptides. By optimizing the proportion of these two materials, extremely high sensitivity and selectivity are achieved in analyzing the standard glycopeptides/nonglycopeptides mixture solutions. Since the washing step is not necessary for these conditions, the enrichment process is simplified and the recovery efficiency of target glycopeptides reaches 90%. Finally, this approach is successfully applied to analyze human serum with the sample volume as little as 1 渭L, in which 147 different N-glycosylation peptides within 66 unique glycoproteins are identified. All these performances by the synergistic enrichment are much better than employing one specific enrichment agent alone.