Driven Translocation of Polynucleotides Through an Aerolysin Nanopore

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• 作者：Chan Cao ; Jie Yu ; Ya-Qian Wang ; Yi-Lun Ying ; Yi-Tao Long
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：May 17, 2016
• 年：2016
• 卷：88
• 期：10
• 页码：5046-5049
• 全文大小：271K
• 年卷期：0
• ISSN：1520-6882

文摘

Aerolysin has been used as a biological nanopore for studying peptides, proteins, and oligosaccharides in the past two decades. Here, we report that wild-type aerolysin could be utilized for polynucleotide analysis. Driven a short polynucleotide of four nucleotides length through aerolysin occludes nearly 50% amplitude of the open pore current. Furthermore, the result of total internal reflection fluorescence measurement provides direct evidence for the driven translocation of single polynucleotide through aerolysin.