Double-Strand Displacement Biosensor and Quencher-Free Fluorescence Strategy for Rapid Detection of MicroRNA

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• 作者：Rong Liao ; Kui He ; Chunyan Chen ; Xiaoming Chen ; Changqun Cai
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：April 19, 2016
• 年：2016
• 卷：88
• 期：8
• 页码：4254-4258
• 全文大小：327K
• 年卷期：0
• ISSN：1520-6882

文摘

We describe a facile quencher-free fluorescence strategy for rapid detection of microRNAs (miRNAs) by using a novel double-strand displacement sensor. The sensor is designed with an outstanding 2-aminopurine (2-AP) fluorophore as a probe and a predesigned cDNA, which can completely complement the target miRNA and partly complement the 2-AP probe. When the target miRNA is added, the cDNA can be competed off from the cDNA\2-AP probe duplex, thereby forming a cDNA\RNA heteroduplex. The free 2-AP probe induces an increase in the fluorescent signal. A limit of detection of 5 nM and a wide linear range from 5 to 1000 nM (R² = 0.9971) are achieved by this assay. The rapid detection strategy can be accomplished within 2 h without expensive nanoparticles and complicated instruments for the whole procedure, thus, offering a significant potential for clinical application.