Rational Design of a Reversible pH-Responsive Switch for Peptide Self-Assembly

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• 作者：Yuri Zimenkov ; Steven N. Dublin ; Rong Ni ; Raymond S. Tu ; Victor Breedveld ; Robert P. Apkarian ; and Vincent P. Conticello
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：May 31, 2006
• 年：2006
• 卷：128
• 期：21
• 页码：6770 - 6771
• 全文大小：142K
• 年卷期：v.128,no.21(May 31, 2006)
• ISSN：1520-5126

文摘

Peptide TZ1H, based on the heptad sequence of a coiled-coil trimer, undergoes fully reversible, pH-dependent self-assembly into long-aspect-ratio helical fibers. Substitution of isoleucine residues with histidine at the core d-positions of alternate heptads introduces a mechanism by which self-assembly is coupled to the protonation state of the imidazole side chain. Circular dichroism spectroscopy, transmission electron microscopy, and microrheology techniques revealed that the self-assembly of TZ1H coincides with a distinct coil-helix conformational transition that occurs within a narrow pH range near the pK_a of the imidazole side chains of the core histidine residues.