NMR-Based Microbial Metabolomics and the Temperature-Dependent Coral Pathogen Vibrio coralliilyticus

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• 作者：Arezue F. B. Boroujerdi ; Maria I. Vizcaino ; Alexander Meyers ; Elizabeth C. Pollock ; Sara Lien Huynh ; Tracey B. Schock ; Pamela J. Morris ; Daniel W. Bearden
• 刊名：Environmental Science & Technology
• 出版年：2009
• 出版时间：October 15, 2009
• 年：2009
• 卷：43
• 期：20
• 页码：7658-7664
• 全文大小：287K
• 年卷期：v.43,no.20(October 15, 2009)
• ISSN：1520-5851

文摘

Coral bleaching occurs when the symbioses between coral animals and their zooxanthellae is disrupted, either as part of a natural cycle or as the result of unusual events. The bacterium Vibrio coralliilyticus (type strain ATCC BAA-450) has been linked to coral disease globally (for example in the Mediterranean, Red Sea, Indian Ocean, and Great Barrier Reef) and like many other Vibrio species exhibits a temperature-dependent pathogenicity. The temperature-dependence of V. coralliilyticus in regard to its metabolome was investigated. Nuclear magnetic resonance (NMR) spectra were obtained of methanol−water extracts of intracellular metabolites (endometabolome) from multiple samples of the bacteria cultured into late stationary phase at 27 °C (virulent form) and 24 °C (avirulent form). The spectra were subjected to principal components analysis (PCA), and significant temperature-based separations in PC1, PC2, and PC3 dimensions were observed. Betaine, succinate, and glutamate were identified as metabolites that caused the greatest temperature-based separations in the PC scores plots. With increasing temperature, betaine was shown to be down regulated, while succinate and glutamate were up regulated.