Synthesis of Three Novel Anionic Gemini Surfactants and Comparative Studies of Their Assemble Behavior in the Presence of Bovine Serum Albumin
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- 作者：Yu-Shu Ge ; Shu-Xin Tai ; Zi-Qiang Xu ; Lu Lai ; Fang-Fang Tian ; Dong-Wei Li ; Feng-Lei Jiang ; Yi Liu ; Zhi-Nong Gao
- 刊名：Langmuir
- 出版年：2012
- 出版时间：April 10, 2012
- 年：2012
- 卷：28
- 期：14
- 页码：5913-5920
- 全文大小：510K
- 年卷期：v.28,no.14(April 10, 2012)
- ISSN：1520-5827
文摘
Three novel anionic sulfonate gemini surfactants, sodium 4,4鈥?(10,19-dioxo-9,11,18,20-tetraazaoctacosane-9,20-diyl) dibenzenesulfonate (Surfactant I), sodium 4,4鈥?(12,21-dioxo-11,13,20,22-tetraazadotriacontane-11,22-diyl) dibenzenesulfonate (Surfactant II), and sodium 4,4鈥?(14,23-dioxo-13,15,22,24-tetraazahezatriacontane-13,24-diyl) dibenzenesulfonate (Surfactant III), with different lengths of hydrophobic tail have been synthesized, and their assembly behavior in the presence of bovine serum albumin (BSA) has been studied using spectral methods and molecular modeling methods at physiological pH and 298 K. Critical micelle concentrations (CMCs) of the three surfactants have been determined by surface tension measurements. Despite the obvious decrease of CMC with the increase of tail length, fluorescence spectra have shown much closer CAC in the presence of BSA. Surfactant II shows the highest CAC of 3.19 脳 10鈥? mol L鈥? compared with the other two. The polarity of the microenvironment in BSA鈥搒urfactant systems has been investigated using pyrene as the probe. In addition, far-UV CD spectra studied the change of the secondary structure content of BSA caused by the three surfactants. The features of the assembly behavior were discussed by three concentration regions. Surfactant II could unfold the protein much more efficiently than the other two surfactants at low concentration, but at high concentration, the change of the secondary structure and the formation of hydrophobic microenvironment show a direct relationship to the length of the hydrophobic tail with the increase of the surfactant concentration.