Structural Model of the Amyloid Fibril Formed by 2-Microglobulin #21-31 Fragment Based on Vibrational Spec
详细信息 查看全文
- 作者：Hirotsugu Hiramatsu ; Yuji Goto ; Hironobu Naiki ; and Teizo Kitagawa
- 刊名：Journal of the American Chemical Society
- 出版年：2005
- 出版时间：June 8, 2005
- 年：2005
- 卷：127
- 期：22
- 页码：7988 - 7989
- 全文大小：65K
- 年卷期：v.127,no.22(June 8, 2005)
- ISSN：1520-5126
文摘
A structural model of amyloid fibril formed by the #21-31 fragment of 
2-microglobulin is proposed from microscope IR measurements on specifically 13C-labeled peptide fibrils and Raman spectra of the dispersed fibril solution. The 13C-shifted amide frequency indicated the secondary structure of the labeled residues. The IR spectra have demonstrated that the region between F22 and V27 forms the core part with the extended -sheet structure. Raman spectra indicated the formation of a dimer with a disulfide bridge between C25 residues.
2-microglobulin is proposed from microscope IR measurements on specifically 13C-labeled peptide fibrils and Raman spectra of the dispersed fibril solution. The 13C-shifted amide frequency indicated the secondary structure of the labeled residues. The IR spectra have demonstrated that the region between F22 and V27 forms the core part with the extended -sheet structure. Raman spectra indicated the formation of a dimer with a disulfide bridge between C25 residues.