Detection of Specific Sequences in RNA Using Differential Adsorption of Single-Stranded Oligonucleotides on Gold Nanoparticles

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• 作者：Huixiang Li and Lewis Rothberg
• 刊名：Analytical Chemistry
• 出版年：2005
• 出版时间：October 1, 2005
• 年：2005
• 卷：77
• 期：19
• 页码：6229 - 6233
• 全文大小：181K
• 年卷期：v.77,no.19(October 1, 2005)
• ISSN：1520-6882

文摘

We have used the disparity in adsorption rates for single-and double-stranded RNA on ionically coated gold nanoparticles suspended in a colloid to design a rapid sequence identification assay. Unlabeled target RNA and aprobe sequence are mixed prior to exposure to the goldnanoparticles to enable efficient hybridization. We havedesigned assays based on either color changes or fluorescence that are sensitive to a few picomoles of target.Single-base mutations on RNA sequences can be detectedeven in complex oligonucleotide mixtures. The assayrequires less than 10 min so that RNA degradationproblems are avoided.